International Sugar Journal

Production of healthy seed cane in Tucumán, Argentina* [Full subscriber]

Abstract Since 2001, the ‘Estación Experimental Agroindustrial Obispo Colombres’ (EEAOC) has been working on the ‘Vitroplantas Project’. On average 70 000 sugarcane plantlets of commercial varieties are produced through in vitro meristem culture in order to eliminate systemic diseases caused by bacteria and viruses. The sanitation of plant material is achieved through in vitro culture of apical meristems from donor plants previously hot-water-treated and grown for 3 years under greenhouse conditions with anti-aphid screens. Systemic diseases are detected in both meristem donor plants and micropropagated plantlets using different molecular diagnostic techniques. It is well known that in vitro plant tissue…

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