International Sugar Journal

Optimizing conditions for dextranase application of sugarcane mixed juice using response surface methodology [Full subscriber]

Not only does the formation of dextran results in expensive sucrose losses, but also the high-viscosity associated with this polysaccharide often interrupts normal processing operations. The optimization of dextranase to hydrolyze dextran in sugarcane mixed juice (MJ) was carried out using response surface methodology (RSM). A Box–Behnken factorial design with four factors and three levels, including five replicates at the centre point, was used to determine the influence of enzyme concentration, pH, temperature, and reaction time on the hydrolysis of dextran. Considering practice manufacturing process in sugar mills, the optimum operational conditions were: enzyme concentration 5-10 mg/kg (dextranase activity 30000…

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