International Sugar Journal

Molecular detection and identification of sugarcane white leaf phytoplasma in Vietnam [Full subscriber]

The symptom of white leaf disease (SCWL) has been observed on sugarcane in Southern Vietnam recently. The presence of polymorphic phytoplasma cells in phloem sieve tube elements was confirmed by transmission electron microscopy. A nested polymerase chain reaction assay using phytoplasma-universal primer pairs P1/P7 and R16F2n/R16R2 amplified products of 1,113 bp from the sugarcane white leaf symptomatic canes; whereas no amplicon was detected from symptomless samples collected from Northern Vietnam. Sequence analysis of the 16S rDNA products indicated that the phytoplasma strain (SCWL-DNVN) associated with sugarcane white leaf disease in Southern Vietnam shared 100% sequence similarity with the SCWL phytoplasma…

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