International Sugar Journal

Kinetic behaviour of invertase in the hydrolysis of sucrose in complex sugarcane juices [Full subscriber]

The kinetics of invertase from S. cerevisiae in complex systems of sugarcane was studied as a base of knowledge to produce juices and syrups rich in fructose directly from sugarcane juices that can compete with high fructose corn syrups. For this, solutions of pure sucrose as a model, clarified sugar cane juice, and clarified and purified cane juice, were enzymatically treated with invertase and then hydrolyzed to analyze the kinetics of the reaction using, the parameters of the Michaelis-Menten model and considering invertase inhibition due to excess substrate. The results showed that the Km, Vmax, and Ki in the pure…

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