International Sugar Journal

Isolation and analysis of microbial contaminants from Louisiana raw sugarcane factories [Full subscriber]

Abstract A persistent challenge during raw sugar production is the mitigation of the growth of microbes that consume sucrose and produce polysaccharides. Here, a sugarcane-based growth medium, CJ+, was developed and utilized along with molecular microbiology approaches to isolate and identify microorganisms present in Louisiana raw sugarcane factories. In addition to the well-known Leuconostoc, several other bacterial and yeast genera were identified. All these microbes contribute to sucrose degradation, with some consuming greater quantities as measured by high performance liquid chromatography (HPLC). Exopolysaccharide (EPS) production was observed to vary between isolates, and EPS production by Leuconostoc and Gluconobacter isolates was…

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