International Sugar Journal

How to use and interpret the results from a high performance liquid chromatography system at a sugarcane factory [Full subscriber]

Abstract In 2015, a sugarcane factory established and operated the first High Performance Liquid Chromatography (HPLC) system in Louisiana. Although many HPLC systems exist, the factory opted for an ion chromatography (anion exchange) system with integrated pulsed amperometric detection. A gradient HPLC method was established to measure mannitol, glucose, fructose, and sucrose in a run time of 10 min. For the best accuracy, separate and higher dilutions are needed to quantitate sucrose due to its considerably higher concentration in sugar products (except molasses). Since an analytical technique is only useful if the results can be interpreted properly, training was essential…

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