International Sugar Journal

Genetic diversity of Sugarcane yellow leaf virus in a sugarcane selection plot in Guadeloupe (FWI) † [Full subscriber]

The genetic diversity of Sugarcane yellow leaf virus (SCYLV) was investigated in a sugarcane selection plot in Guadeloupe to determine the incidence of the different known virus genotypes (BRA-PER, CUB and REU) in a breeding progeny. Four F1 leaves were randomly collected from each of 154 sugarcane clones and tested for the presence of SCYLV by tissue blot immunoassay (TBIA). The leaf samples were stored at -80°C until total RNA was extracted from SCYLV-infected leaves. The virus genotypes were determined by RT-PCR and primer pairs specific to each virus genotype. Eighty-two percent of the tested leaves were infected by SCYLV…

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