International Sugar Journal

Evaluation of genetic response of sugarcane (Saccharum species hybrids) genotypes with varied concentration of cytokinins for rapid in vitro mass multiplication [Full subscriber]

Abstract In a lab experiment, the efficacy of six different combinations of cytokinins 6-Benzyl amino purine (BAP) and kinetin (Kin) on in vitro multiplication of four sugarcane genotypes were evaluated. Findings indicated that MS medium supplemented with BAP (0.2 mg/l) + Kin (0.015 mg/l) produced the best growth response in genotype Co 86032 with superior auxiliary bud emergence, and shoot establishment than the other genotypes.  MS medium with BAP (0.5 mg/l) + Kin (0.015 mg/l) proved optimum for genotypes VCF 0517, CoVC 09-61-07, and CoVC 10-43-06. However, multiple shoot induction characters varied widely among the four sugarcane genotypes. The lowest…

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