International Sugar Journal

Effects of chilling stress on protein and related gene expression in chloroplasts of sugarcane* [Full subscriber]

Abstract The effects of chilling stress on chloroplasts at protein and mRNA levels to provide a reference for further investigation on the molecular mechanism of chilling stress in sugarcane was investigated. Sugarcane varieties GT28 (strong cold resistant) and ROC22 (weak cold resistant) were used as plant materials, and treated under 9 d, 15 d and 24 d of chilling stress, respectively. The chloroplast protein was extracted and used for electrophoresis analysis, and expressions of the genes were analysed using real-time PCR. Electrophoresis analysis showed that, for both sugarcane varieties, the relative content of chloroplast protein decreased with increase in chilling…

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