International Sugar Journal

Effectiveness of different sources of nitrogen in MS media for rapid in vitro shoot initiation of sugarcane (Saccharum officinarum L.) genotypes. [Full subscriber]

Abstract Tissue culture techniques are important tools of crop improvement and rapid multiplication of sugarcane clones. Nitrogen is essential not only for growth, but also for morphogenesis in tissue culture, which is markedly influenced by the availability of nitrogen and the form in which it is presented in the plant. In a laboratory experiment with sugarcane genotypes Co 86032, CoVC 15-22-05, CoVC 15-22-26 and CoVC 18061, the efficacy of different sources of nitrogen; namely ammonium sulphate, tri ammonium citrate and supplement for MS (Murashige and Skoog) medium were evaluated. There was no consistent response from genotypes from the different N…

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