International Sugar Journal

Comparison of RSD assays for diagnosis and screening varieties for resistance † [Full subscriber]

Ratoon stunting disease (RSD), caused by the bacterium Leifsonia xyli subsp. xyli, has no specific external symptoms and internal symptoms are often unreliable. Immunoassays are widely used to diagnose the disease because they are sensitive, relatively inexpensive and are suitable for bulk screening. The most widely used immunoassays for RSD diagnosis are the enzyme-linked immunosorbent assay (ELISA) and the stalk tissue blot immunoassay. Phase-contrast microscopy is also used for diagnosis but one person can only process 100–200 samples in a day. These assay methods all use extracts from stalks of mature cane. Collecting stalks from mature crops of cane is…

Login or sign up

Create an account

Lost your password?